AGN_194310_MS_19863_MedChemExpress

MS Report File ..322 2016-03-22 09-59-06\\BIZ2016-322-WJ3-1.D Tgt Mass (CHM): Injection Date : 22 Mar 16 11:34 am +0800 Seq. Line : 23 Sample Name : BIZ2016-322-WJ3-1 Location : P1-E-08 Acq. Operator : Su Xiao Ying(LCMS-02) Inj : 1 Spec. Reported : MS Integration Inj Volume : 8 ul Acq. Method : D:\\Aglient 1260\\Data\\20160322\\20160322 2016-03-22 09-59-06\\100-1000MS+3MIN- Analysis Method : D:\\AGLIENT 1260\\DATA\\20160322\\20160322 2016-03-22 09-59-06\\100-1000MS+3MIN- Catalog No : HY-16681 Batch#19863 A-RP-134 Method Info : HY-365_5HO1RS,M,A-RP-108，210nm,23min DAD1 B, Sig=214,4 Ref=off 2.3832.5562.938mAU100000.5 DAD1 C, Sig=254,4 Ref=off mAU100000.51 MSD1 TIC, MS File ES-API, Pos, Scan, Frag: 501000001.9680.3251.5811.6201.6770.2350.8300.8750.9240.5921.1051.1623.09611.522.533.096min2.3832.5492.9371.522.3942.52.67033.1703.223min2.93300.1500.6690.7200.7571.2341.3441.3961.7471.8101.8660.434500002.2053.0120.511.522.53 MSD1 425, EIC=424.7:425.7 (D:\\AGLIENT 1260\\DATA\\20160322\\20160322 2016-03-22 09-59-06\\BIZ2016-322-WJ3-1.D) ES-API, 200000.51 Ion 1, MSD1 1,Target Mass 0 +H Positive, EIC=0.7:1.7 0-0.10.51 Ion 1, MSD1 1,Target Mass 0 +H Positive, EIC=0.7:1.7 1.522.531.522.533.098minminmin0-0.10.511.522.53min Integration Results for DAD1 B, Sig=214,4 Ref=off RetTim Width Area Height Area%|-------|--------|--------------|--------------|--------| 2.38 0.07 829.12 189.77 12.72 2.56 0.04 48.79 20.53 0.75 2.94 0.05 64.25 20.31 0.99 3.10 0.05 5576.03 1869.27 85.55 Integration Results for DAD1 C, Sig=254,4 Ref=off RetTim Width Area Height Area%|-------|--------|--------------|--------------|--------| 2.38 0.07 849.05 194.64 14.26 2.55 0.05 97.37 31.74 1.64 2.94 0.04 36.78 15.30 0.62 3.10 0.05 4969.32 1703.52 83.48HY-LCMS-02 3/22/2016 1:02:20 PM Su Xiao Ying(LCMS-02)Page 1 of 7

3.500MS ReportRet. Time: 0.15 <<<< POSITIVE SPECTRA >>>> ES-API Positive 102.2 110.1Ret. Time: 0.23 ES-API Positive 102.21200100080012001000800 183.1 183.1600400 101.1600 223.1 110.1 111.1 219.1400 117.1 235.0 101.2 142.1 158.1 109.4 117.1 200.10100150200m/z100200300m/zRet. Time: 0.33 ES-API Positive 102.2Ret. Time: 0.43 ES-API Positive 102.2300025002500200020001500 110.11500 110.11000 151.1 183.01000 110.8200 167.1 102.8 111.1 219.1 103.25000100200300m/z0100200300m/zRet. Time: 0.59 ES-API Positive 102.2Ret. Time: 0.67 ES-API Positive 102.214001200100080060012001000 110.2800 110.1600 223.1 223.1 200.1 112.3 118.2 182.9 101.2 117.10100150200250300m/z0100150200m/zHY-LCMS-02 3/22/2016 1:02:20 PM Su Xiao Ying(LCMS-02) 110.9 116.2 142.0200 224.1200 101.1Page 2 of 7

219.1400 183.0400 112.2 128.2500 183.1 223.3 352.3200 219.1 223.2MS ReportRet. Time: 0.72 ES-API Positive 102.2Ret. Time: 0.76 ES-API Positive 102.21200120010001000800600 223.1 183.0 110.1800600 110.1 101.2 112.2 219.1 101.24004002000 183.1 200.1 142.0 224.1 224.2 111.10100200300m/z 202.1100200300m/zRet. Time: 0.83 ES-API Positive 102.2Ret. Time: 0.88 ES-API Positive 102.2140012001000140012001000 110.2800600400 101.1800 223.2 183.1 112.3 219.1 111.0 117.1 104.120002000100150200m/z 117.1 219.1400 183.0 223.3600 110.2100200300m/zRet. Time: 0.92 ES-API Positive 102.2Ret. Time: 1.11 ES-API Positive 102.2140012001000800600400140012001000800600 223.2 183.0 110.1 110.2 112.2 101.2 112.2400 352.4 183.0 101.1 219.1 117.1 202.1 333.220002000100200300m/z100150200HY-LCMS-02 3/22/2016 1:02:20 PM Su Xiao Ying(LCMS-02)Page 3 of 7

219.1m/z 223.1 333.1200 110.8 219.1 223.1MS ReportRet. Time: 1.16 ES-API Positive 102.2Ret. Time: 1.23 ES-API Positive 102.2 110.11400120016001400120010001000800 110.1800 223.2600 101.1 201.9 183.1400 219.1 224.2 110.9 112.2 101.1 112.2 183.04002000 118.32000100150200m/z 202.0 223.3600100150200250m/zRet. Time: 1.34 ES-API Positive 102.2Ret. Time: 1.40 ES-API Positive 102.2180016001400120010001600140012001000 110.1 223.1 224.2800600 101.2800 223.3 112.24002000 219.3 111.2 105.14002000 112.1600 110.0100200300m/z100200300m/zRet. Time: 1.58 ES-API Positive 102.2Ret. Time: 1.62 ES-API Positive 102.2200017501500125010002000175015001250 110.1 223.2 110.17505002500 101.2 112.1 116.2 254.2 270.1 274.8750500 548.5 223.21000 254.1 270.2 111.1 112.2 200.1 224.22500200400m/z 333.2100200300400500m/zHY-LCMS-02 3/22/2016 1:02:20 PM Su Xiao Ying(LCMS-02)Page 4 of 7

549.5 548.5 172.3 172.1 333.2MS ReportRet. Time: 1.68 ES-API Positive 102.2Ret. Time: 1.75 ES-API Positive 102.222502000175015001250200017501500 105.11250 105.1 223.2100075050025001000 270.1 274.9 548.5 110.1 110.1 234.3 111.2 549.5 111.1 467.45002500 274.7 226.1 201.9 253.9 321.3 338.2100200300400500m/z 226.2 333.0100200300400500m/zRet. Time: 1.81 ES-API Positive 102.2Ret. Time: 1.87 ES-API Positive 102.2225020001750225020001750 105.115001250100075015001250 223.2 105.11000 270.2750 548.5 112.2 274.8 338.1 467.4 112.3 223.250025005002500 200.1 539.4 234.9 103.2 321.2 186.1 321.1 338.1 111.6 469.2 226.1100200300400500m/z 254.2100200300400500m/zRet. Time: 1.97 ES-API Positive 102.2 234.2Ret. Time: 2.20 ES-API Positive102.2 105. 1225020001750150012501000750 105.225002000 223.2 467.4 223.21500 274.4 270.31000 468.4 254.9 416.2 415.3 468.3 467.3 539.4 110.2 548.55002500 227.0 228.2 318.2 333.2 224.1 200.1 276.3 103.2 302.40200400m/z200400600m/zHY-LCMS-02 3/22/2016 1:02:20 PM Su Xiao Ying(LCMS-02) 333.1 350.3 369.3 200.1 251.2500 279.2Page 5 of 7

540.3 548.6 549.6 548.5750 223.3MS ReportRet. Time: 2.39 ES-API Positive 572.3Ret. Time: 2.67 ES-API Positive 338.4 572.3 573.3 105.2 282.3 339.43500030000250002000015000100001400012000100008000 102.2 573.2600040000200400m/z 105.20200400m/zRet. Time: 2.93 ES-API Positive 102.2Ret. Time: 3.01 ES-API Positive 102.212000100008000800060006000 105.14000 223.2 282.3 223.2 350.3 350.4 103.2 200.1 328.4 103.20200400m/z 572.20200400m/zRet. Time: 3.17 ES-API Positive 102.2Ret. Time: 3.22 ES-API Positive 102.21400012000100008000100008000 105.1 105.1600040006000 223.24000 282.3 350.3 408.3 425.2 223.2 228.3 244.2 103.2 328.40200400m/z0200400m/zHY-LCMS-02 3/22/2016 1:02:20 PM Su Xiao Ying(LCMS-02) 226.3 338.32000 103.22000 282.3 328.32000Page

425.22000 282.34000 105.1 328.3500020006 of 7

595.2 594.2 223.2 574.3 360.4 574.2MS ReportRet. Time: 3.50 ES-API Positive 102.2Ret. Time: 3.10 <<<< POSITIVE SPECTRA >>>> Positive 102.21400012000100008000100008000 105.16000 105.160004000200004000 223.3 223.2 282.3 310.4 350.3 350.40200400m/z200400m/zHY-LCMS-02 3/22/2016 1:02:20 PM Su Xiao Ying(LCMS-02) 328.3 408.3 425.2 103.2 103.2 228.32000 282.3Page 7 of 7